Neutrophil extracellular trap components increase the expression of coagulation factors.

Neutrophil extracellular traps (NETs) represent an important link between inflammation and thrombosis. Here, the present study aimed to investigate the influence of the NET components, DNA and histone H4, on hemostatic gene expression. A further aim was to confirm the influence of H4 on the expression of tissue factor (TF) and investigate a potential effect of DNA, and to test the involvement of miR-17/92 and its paralog miR-106b-25 in this regulation. In HepG2 cells, the mRNA levels of factor VII and factor XII, which are crucial in the activation of the coagulation cascade, and of serpin family F member 2 (encoding α2-antiplasmin) were significantly upregulated by DNA and H4; while the mRNA levels of factor V, which is essential for thrombin generation of protein S, a cofactor of protein C that also has the ability to inhibit the factor X activation pathway, and of serpin family C member 1 (encoding antithrombin, the main endogenous anticoagulant) were significantly upregulated only by H4. H4 and DNA also provoked an increase in hepatocyte nuclear factor 4α (HNF4A) mRNA expression that could be responsible for the increase in the expression of certain coagulant factors. In THP-1 cells, it was also demonstrated that H4 caused an increase in TF mRNA while decreasing several of the microRNAs (miRNA/miRs) of the cluster miR-17/92, which may in part explain the increase in the expression of TF. The present results suggest the ability of NET components to alter the hemostatic balance and a possible involvement of HNF4α and miRNAs in this regulation.

Evaluación nutricional y de tensión arterial en niños de dos escuelas de población vulnerable de Cutral Co y Plaza Huincul. Estudio cuantitativo y cualitativo / Nutritional status and blood pressure assessment in vulnerable children from two schools in Cutral Co and Plaza Huincul. A quantitative and qualitative study

Introducción. La situación nutricional de los niños constituye un conocimiento fundamental para la programación de intervenciones de atención primaria en el contexto de la actual epidemia de obesidad y de aumento de la prevalencia de síndrome metabólico en la infancia. Por ello, se realizó una evaluación nutricional y de tensión arterial de alumnos de dos escuelas con alta vulnerabilidad social, junto con un análisis cualitativo de los resultados, y se buscó conocer las opiniones de los integrantes de la comunidad para generar un conocimiento útil para la programación de intervención en salud a nivel local. Población y métodos. Estudio trasversal en escuelas seleccionadas intencionalmente, con análisis de los resultados mediante grupos focales. Se utilizaron estándares de la Organización Mundial de Salud (OMS) para peso y talla, del National Center for Health Statistics (NCHS) para índice de masa corporal (IMC), de los National Institutes for Health (NIH) para tensión arterial y las referencias de Fernández J. et al. para circunferencia de cintura. Resultados. De 361 niños evaluados, 20,8% presentaron sobrepeso; 22,2%, obesidad; y 6,5%, valores de hipertensión. Hubo mayor prevalencia de obesidad en varones (p: 0,039), y no se encontró asociación entre hipertensión y obesidad central con edad y sexo. Representantes de la comunidad describieron los contextos sociales que influían en la situación encontrada. Conclusiones. Se definió que esta población presentaba una situación de malnutrición, con alta prevalencia de obesidad. La comunidad analizó los resultados focalizando los puntos a tener en cuenta para la intervención.

Introduction. Knowing the nutritional status of children is critical to plan primary health care interventions in the setting of the present obesity epidemic and the great increase in the prevalence of childhood metabolic syndrome. For this reason, nutritional status and blood pressure were assessed among children from two schools of high social vulnerability, results were qualitatively analyzed, and community members feedback was obtained in order to be fully aware of the situation and accordingly plan local health interventions. Population and methods. Cross-sectional study conducted at purposively selected schools, with results analyzed using focus groups. The following standards were used: the World Health Organization (WHO) growth standards for weight and height; the National Center for Health Statistics (NCHS) for body mass index (BMI); the National Institutes of Health (NIH) for blood pressure; and Fernández J., et al. standards for waist circumference. Results. Three hundred and sixty one children were evaluated; of them, 20.8% were overweight, 22.2% were obese and 6.5% were hypertensive. Obesity was more prevalent in boys (p: 0.039), and no relationship was observed between hypertension and central obesity with age and sex. The social factors that influence this situation were described by community representatives. Conclusions. It was established that this population was malnourished and had a high prevalence of obesity. Results were analyzed by community members who focused on the items to be considered for the intervention

Nutritional status and blood pressure assessment in vulnerable children from two schools in Cutral Co and Plaza Huincul: a quantitative and qualitative study.

INTRODUCTION: Knowing the nutritional status of children is critical to plan primary health care interventions in the setting of the present obesity epidemic and the great increase in the prevalence of childhood metabolic syndrome. For this reason, nutritional status and blood pressure were assessed among children from two schools of high social vulnerability, results were qualitatively analyzed, and community members feedback was obtained in order to be fully aware of the situation and accordingly plan local health interventions. POPULATION AND METHODS: Cross-sectional study conducted at purposively selected schools, with results analyzed using focus groups. The following standards were used: the World Health Organization (WHO) growth standards for weight and height; the National Center for Health Statistics (NCHS) for body mass index (BMI); the National Institutes of Health (NIH) for blood pressure; and Fernández J., et al. standards for waist circumference. RESULTS: Three hundred and sixty one children were evaluated; of them, 20.8% were overweight, 22.2% were obese and 6.5% were hypertensive. Obesity was more prevalent in boys (p: 0.039), and no relationship was observed between hypertension and central obesity with age and sex. The social factors that influence this situation were described by community representatives. CONCLUSIONS: It was established that this population was malnourished and had a high prevalence of obesity. Results were analyzed by community members who focused on the items to be considered for the intervention.

Nutritional status and blood pressure assessment in vulnerable children from two schools in Cutral Co and Plaza Huincul: a quantitative and qualitative study. / Nutritional status and blood pressure assessment in vulnerable children from two schools in Cutral Co and Plaza Huincul: a quantitative and qualitative study.

INTRODUCTION: Knowing the nutritional status of children is critical to plan primary health care interventions in the setting of the present obesity epidemic and the great increase in the prevalence of childhood metabolic syndrome. For this reason, nutritional status and blood pressure were assessed among children from two schools of high social vulnerability, results were qualitatively analyzed, and community members feedback was obtained in order to be fully aware of the situation and accordingly plan local health interventions. POPULATION AND METHODS: Cross-sectional study conducted at purposively selected schools, with results analyzed using focus groups. The following standards were used: the World Health Organization (WHO) growth standards for weight and height; the National Center for Health Statistics (NCHS) for body mass index (BMI); the National Institutes of Health (NIH) for blood pressure; and Fernández J., et al. standards for waist circumference. RESULTS: Three hundred and sixty one children were evaluated; of them, 20.8

were overweight, 22.2

were obese and 6.5

were hypertensive. Obesity was more prevalent in boys (p: 0.039), and no relationship was observed between hypertension and central obesity with age and sex. The social factors that influence this situation were described by community representatives. CONCLUSIONS: It was established that this population was malnourished and had a high prevalence of obesity. Results were analyzed by community members who focused on the items to be considered for the intervention.